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Custom software packages were written in-house in order to track sample submissions, track fractions collected, make selections of ractions for loop injection MS validation, label samples, and generate chromatographic and mass spectrometry reports for return to the synthetic chemist Sample submission begins with an Intranet-based log-in system (as shown in Figure 111) The chemist provides laboratory notebook designation (for sample tracking purposes), structure, and MW (if available) for the desired component(s), weight of the crude material to be puri ed (for the selection of column size), and any comments regarding special handling or information relevant to puri cation This log-in system has a manual sample mode in which individual compounds can be entered singly and is also compatible with our company s electronic notebook system so that all data for large library sets can be populated automatically from the electronic notebook.

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Samples submitted via the electronic notebook appear on the worksta- Using Barcode generator for NET framework Control to enerate, create bar code image in Visual Studio NET applicationstion for each of the puri cation chemists in HTP in a custom software package henever possible, samples from multiple sources are grouped for puri cation This can be done whenever the optimal wavelength for fraction collection trigger and the optimal gradient elution method are deemed identical for multiple sample sets Doing so increases ef ciency of the overall process The software allows the assignment of puri cation parameters to a speci c preparative scale HPLC Upon completion of puri cation, data les and fraction collection racks are passed on for loop injection MS of fractions selected by the puri cation chemist.

This system of UVtriggered fraction collection followed by MS validation has been the mainstay puri cation technique for HTP at Abbott for several years It was adopted on the principle that several relatively inexpensive yet slow HPLCs feeding into a single more costly but fast MS give good value and allow for expansion We have thus succeeded in accommodating initially two and presently four preparative UV/ELSD-triggered HPLCs At this point custom software allows importation of chromatograms and correlated mass spectrometry data to be reviewed by the puri cation chemist (as shown in Figure 112) Those fractions corresponding to the desired component(s) are selected for sample labeling and dry-down The sample, along with a Puri cation Report (as shown in Figure 113), is returned to the submitting chemist.

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HPLC (MS) In addition to the UV or ELSD triggered HPLC instruments described above, an MW-triggered HPLC instrument is available in our HTP facility This is commercially known as the Agilent (Wilmington, DE) 1100 series LC/MSD, which includes a PDA detector and mass spectrometer and a Gilson 215 liquid-handler for injection and fraction collection An Antek (Houston, TX) 8060 nitrogen speci c detector (Chemiluminescent Nitrogen Detection CLND) was integrated into the system in order to achieve on-line determinations of sample weights during puri cation Custom macros as well as a custom data browser were written for sample tracking and data viewing,27 which includes CLND quantitation information made available in the nal client report The chromatography column employed on this system is a Phenomenex LunaR C8, 5-m particle size column of 50 212 mm dimensions, used for the puri cation of 10 to 50 mg HTOS libraries.

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Standard gradient elution methods used are 0 100% MeOH : 01% aqueous TFA, 10 100% MeOH : 01% aqueous TFA, 20 100% MeOH : 01% aqueous TFA, 50 100% MeOH : 01% aqueous TFA, with methanol being used in lieu of acetonitrile so that solvent does not interfere with on-line CLND quantitation resultsRelated: .

Every application begins its life in source code form, and to make an executable ou need to compile it for your architecture. When you compile software, you have to define certain parameters that the source code is compiled under. This could be software optimization, file installation locations, or options to enable or disable certain software functionality. Once compiled, the software is installed into the system to be able to run on its own. This process is repeated for each iteration of the software release that you wish to install. However, this process does not control the software versioning, and there is a great danger that older library versions of software could conflict with the software when you upgrade. The RPM system allows you to semi-automate this process with the definition of a spec file (specification file). The spec file defines the location of the source code, preparing the code for compilation and installation of the source code in one manageable package. Inside the spec file is also a definition of the version of the code and the location of all files the RPM contains. This allows for updates to overwrite (effectively removing the old software and installing the new), negating the impact of the traditional configure, compile, and install with source code. The architecture of an RPM package can be a ubset of a general architecture; for example, Intel can be i386 (386-based, works on all Intel-based machines). The i586/i686/Athlon architectures will usually contain specific optimizations for the higher Intel/AMD processors. encoder todeploy bar code in font. Barcode barcode library for .Related: 

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Pre-puri cation analytical SFC/ MS/CLND allows the triage of samples for puri cation, and an in-house software package analyzes data for predicted quality based on an evaluation of UV and MS data for he potential of co-eluting peaks during puri cation. This same software package selects a collection time window for puri cation, which is necessary to limit the number of fractions per sample. This system accommodates the puri cation of samples up to 50 mg. up to 50 mg. PDF-417 2d Barcode Drawer In .Related: 

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